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phospho stat6 cst  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho stat6 cst
    Phospho Stat6 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho stat6 cst/product/Cell Signaling Technology Inc
    Average 96 stars, based on 218 article reviews
    phospho stat6 cst - by Bioz Stars, 2026-03
    96/100 stars

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    Effects of ESB on STAT3 phosphorylation in RAW 264.7 cells. RAW 264.7 mouse macrophages were stimulated with IL-6 (100 ng/mL, A, B) or IL-4 (100 ng/mL, C, D) in the presence or absence of ESB (50 and 100 μg/mL). After 24 h, the levels of phosphorylated and total STAT3 and STAT6 were analyzed using Western blot analysis. Actin served as a loading control. Representative blot images from three independent experiments are shown (A, C). The ratio of phosphorylated to total protein was quantified by measuring band intensity (B, D). Data are presented as the mean ± SD of three independent experiments. Statistical significance was evaluated using one-way ANOVA with Tukey’s post hoc test ( ## p < 0.01, ### p < 0.001 versus untreated control; ns, not significant; *p < 0.05, ***p < 0.01, ***p < 0.001 versus cells treated with IL-6 or IL-4). ESB, ethanolic extract of Scutellaria baicalensis root; IL, interleukin; STAT3, signal transducer and activator of transcription 3.

    Journal: Journal of Pharmacopuncture

    Article Title: Suppression of Macrophage Migration and M2 Polarization by an Ethanolic Extract of Scutellaria baicalensis Root

    doi: 10.3831/KPI.2025.28.2.144

    Figure Lengend Snippet: Effects of ESB on STAT3 phosphorylation in RAW 264.7 cells. RAW 264.7 mouse macrophages were stimulated with IL-6 (100 ng/mL, A, B) or IL-4 (100 ng/mL, C, D) in the presence or absence of ESB (50 and 100 μg/mL). After 24 h, the levels of phosphorylated and total STAT3 and STAT6 were analyzed using Western blot analysis. Actin served as a loading control. Representative blot images from three independent experiments are shown (A, C). The ratio of phosphorylated to total protein was quantified by measuring band intensity (B, D). Data are presented as the mean ± SD of three independent experiments. Statistical significance was evaluated using one-way ANOVA with Tukey’s post hoc test ( ## p < 0.01, ### p < 0.001 versus untreated control; ns, not significant; *p < 0.05, ***p < 0.01, ***p < 0.001 versus cells treated with IL-6 or IL-4). ESB, ethanolic extract of Scutellaria baicalensis root; IL, interleukin; STAT3, signal transducer and activator of transcription 3.

    Article Snippet: Phospho-STAT3 (Y705) and phospho-STAT6 (Y641) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

    Techniques: Phospho-proteomics, Western Blot, Control